
Ohio University Genomics Facility
The Genomics facility provides rapid and accurate data to the researchers at Ohio University and the outside community in a cost effective manner.
DNA sequencing, $6.50
per reaction. (1-2 day processing)
DNA Array + Processing ~$525.
Bioanalyzer: $24 per 12 RNA samples.
qPCR: $25 per run (optimization runs will not be charged)
The Applied Biosystems 3130xl Genetic Analyzer with 16 capillary through-put, providing automated DNA sequencing, fragment analysis, microsatellite analysis, AFLP, SNP screening and validation.
Design your own set of probes and get them printed, even on a single array, for no extra cost. Agilent uses 60-mer probes, increasing the sensitivity of your reaction 8-fold when compared to 25-mer technology. This system also allows dual color or single color array experiments giving researchers greater flexibility in their experimental design.
The Stratagene Mx3000P QPCR machine offers four channels with with customizable filters for multiplexing. The Mxpro QPCR software is powerful and flexible, providing numerous options for complete data analysis.
The Agilent 2100 Bioanalyzer for quick analysis of DNA, RNA and protein. The microfluidic station can be used to address various questions including, but not limited to, verification of RNA integrity in small samples, PCR product analysis, gene expression analysis, GMO detection, forensic testing and clinical research, for example genotyping of H.pylori.
Software and Training
The facility has dedicated computer workstations with data analysis software, including Vector NTI and Genespring. Special Bioinformatic needs can also be addressed, in conjunction with Ohio University Computer Science Department, using customized programs. The personnel at the Genomics facility will provide training to undergraduate, graduate students and assist researchers with their experimental design.